
Didier Descouens via Wikimedia // CC BY 3.0
Prairie dogs say hello by kissing.


Didier Descouens via Wikimedia // CC BY 3.0
Prairie dogs say hello by kissing.

One question underlies the race for a Zika vaccine: What is this virus, exactly, and how does it work? A new paper in the journal Cell Host & Microbe takes us one step closer to understanding by illuminating the relationship between Zika infection and microcephaly.
Babies with the congenital condition called microcephaly are born with unusually small heads and often have difficulties with brain development. Women who become infected with Zika virus while pregnant are at risk for transmitting the virus to their infants, who may be at higher risk for microcephaly. But just how Zika causes microcephaly has remained something of a puzzle.
To take a closer look, researchers at Stanford University cultured human stem cells in petri dishes and infected them with the virus. As the cells developed into embryos, the team was able to monitor exactly what went wrong with which cells.
Previous studies had focused on neural progenitor cells, which eventually grow into the nervous system. The Stanford team found a vital element in a second type of cell: cranial neural crest cells (CNCC). But where neural progenitor cells are easily killed off by the virus, cranial neural crest cells responded quite differently, secreting two kinds of cytokines, or inflammatory immune response signaling molecules, in reaction to the presence of the virus. That triggered the growth of more new neural cells—and sent the process of brain and skull development off the rails. Some cells split prematurely, and others died. The virus effectively interfered by confusing communication—or “crosstalk,” as the researchers describe it—between cranial neural crest cells and neural progenitor cells.
“Our results suggest that CNCC infection by ZIKV may contribute to associated embryopathies through signaling crosstalk between developing face and brain structures,” they write.
Co-senior author Joanna Wysocka is a chemical and systems biologist at the Stanford University School of Medicine. She says it’s possible that the virus is affecting still other cell types in addition to these two.
“Neural crest cells are one example,” she said in a press statement, “but such mechanisms may also be relevant to other tissues that come in contact with the developing brain during head formation and could be infected by Zika virus.”
Wysocka emphasized that microcephaly is a congenital issue and not a risk for adults who contract the virus.
The team’s findings are “remarkable,” physician Larry Brilliant tells mental_floss. (Brilliant was unaffiliated with the study.) The notion of crosstalk between the cell types is “phenomenally important,” he said, because “they lead to questions of [whether] therapeutic interventions might be possible even after the virus is transmitted.”
Know of something you think we should cover? Email us at tips@mentalfloss.com.
September 29, 2016 – 12:01pm
Earth is nicknamed “the blue planet” for a reason—lakes, rivers, and oceans cover around 71 percent of its surface. Aquariums help us connect with these watery environments, but looking after the creatures that live in these places isn’t always easy. On any given day, the people who take care of water-dwelling animals and plants for a living—they’re known as aquarists—might be asked to swim with sharks, train sea lions, or poke a gassy sea horse.
Like zoos, aquariums get loads of applications whenever an animal care position opens. Therefore, vacancies tend to be filled in short order. “It’s a very, very competitive field,” says Paige Stuart, an aquarist at the Greater Cleveland Aquarium. “Basically everybody wants to do this, so you have to try to get ahead of the game as much as you can.” As a rule, aquariums will expect all applicants to have a degree in biology or a related field. Stuart adds that, on top of this, “You definitely have to be scuba-certified.”
But satisfying those two requirements still might not be enough to get you hired. “It’s harder to get a job without first having a lot of experience,” Sally*, who works at a zoo in the southeastern U.S., notes. “You need the degree, but it’s the experience that’s going to make you better at your job and what will ultimately get you the job in the first place.”
To boost your credentials, Sally recommends volunteering or interning at a local aquarium, zoo, or animal shelter. Applying to pet stores, she adds, might also be a good idea: “They’ll help you get to know the practical side of things, the actual application of your book knowledge.”

Never underestimate those cephalopods. In 2008, an octopus at one German aquarium deliberately short-circuited the lamp above his tank by squirting a jet of water at the fixture. Not to be outdone, an octopus at the Santa Monica Pier aquarium caused a minor flood the next year. After the creature loosened a water-control valve that fed into its own filtration system, 200 gallons of seawater poured out onto the surrounding floor. And last April, yet another octopus pulled off a spectacular escape worthy of Steve McQueen. Staffers at the New Zealand National Aquarium were dumbfounded to discover that their octopus named Inky had crawled out of his tank and squeezed into a six-inch-wide drain. Where did this exit lead? Directly into the Pacific Ocean.
“Believe me, you need to know a lot about plumbing for this job,” Vickie Sawyer, an aquarist at Norwalk’s Maritime Aquarium, observes. Those who work with captive fish and marine animals deal with all manner of filters, valves, and pumps, and the bigger the aquarium, the more elaborate the plumbing. Consider, for instance, the world-famous Georgia Aquarium, which utilizes an expansive network of filtration systems that distributes 10 million gallons of water throughout the facility. To keep things running smoothly, a Life Support System (LSS) team oversees some 225 individual pumps. These highly trained employees are also responsible for monitoring the water temperature and pH level in each tank, among other things.

As most people know, it’s the male seahorse that gives birth. A special pouch on his stomach makes this amazing feat possible. When these fish mate, the females deposit their eggs into the pouch. Ten to 25 days later, as many as 2000 babies will come shooting out of this receptacle. Sally tells us that the unusual breeding process makes male sea horses susceptible to an odd medical problem: Excessive gas sometimes accumulates inside of their pouches. To deal with this, an aquarist will need to gently grasp an afflicted male, keeping him just below the water’s surface. Then, Sally explains, careful pressure should be applied to his midsection with a blunt, stick-shaped object (e.g., a plastic pipette). If all goes well, the gas will be released in the form of harmless bubbles.
Every fish that’s given to a seal or sea lion has to be rigorously inspected for quality and health. “We check their eyes, their bodies, and press their stomachs to make sure they aren’t too soft,” Sawyer says. According to Sally, if even a single scale is out of place, the fish can’t be used. “There are a lot of really strict regulations on marine-mammal keeping,” she says. “I used to work with seals and when I did, I spent 90 percent of my time in the kitchen, making sure the fish were perfect. If any little scale was missing, that could mean that some parasite had gotten inside the fish.” When all’s said and done, the potential entrée has to be a restaurant-quality specimen.

So-called “touch tanks” are roofless enclosures that allow guests to reach down and actually touch a small menagerie of aquatic animals. Given the hands-on appeal, such exhibits are quite popular—especially with kids. Unfortunately, visitors sometimes get a little too rough with the display creatures. In order to keep their creatures from getting injured or stressed, aquariums will often enforce a strict “two-finger touch” policy (rather than using the whole hand). It’s also common practice to ask all guests to wash their hands before they approach the tank.

In the words of biologist Toni Loschiavo, “There’s a lot of time, there’s a lot of patience, there’s a lot of care that goes into each animal that you see performing in a show.” An assistant curator at the Mystic Aquarium in eastern Connecticut, Loschiavo helps manage the facility’s Maritime Theater, where trained sea lions dazzle crowds several times a day. Experience has taught her that even the most basic marine mammal stunts are the fruit of some intense labor. For instance, she claims that teaching a pinniped (seal or sea lion) just to lift its flipper on command is a process that can take more than 30 hours.
Most trainers employ a method called “positive reinforcement operant conditioning.” This involves giving the animal a command and then rewarding it—usually with food—for responding appropriately. To inform the creature that it’s done a good job and that a reward is on the way, trainers use a special signal known as a “bridge.” Normally a sight or sound cue of some kind (eg: blowing a whistle), this lets the animal know it has done a good job and prevents it from getting frustrated while its trainer grabs a treat. [PDF]

Myrtle the green sea turtle is arguably the most beloved reptile in Boston. Over the past 46 years, she’s become a fan favorite at the New England Aquarium, where she spends her days eating Brussels sprouts and hanging out with sharks in a gorgeous four-story tank. “She arrived on June 12, 1970 … the year after we opened,” New England Aquarium senior aquarist Sherrie Floyd Cutler told Boston Magazine. Myrtle was acquired from the now-defunct Provincetown Aquarium in exchange for some smaller turtles. Cutler reveals that it’s “very common for aquariums and zoos to trade surplus animals … In terms of conservation, it helps partially to stock exhibits, especially when you’re talking about an endangered species. Myrtle was too big for their tank, so it worked great for us.”
“You have to report every single death,” Sally tells mental_floss. Oftentimes, the staff veterinarian(s) will perform a necropsy (an animal autopsy) in order to ascertain why the creature died. Information gleaned from this procedure can be invaluable; if it turns out that a certain fish was killed by some kind of disease, the necropsy might help the staff fight the outbreak before it can spread further and potentially wipe out an entire tank.
Just ask Ruby Banwait, a fish specialist at the Vancouver Aquarium [PDF]. “Much of the work of an aquarist is cleaning—cleaning glass, cleaning walls, cleaning gravel,” she says. “This is important not only for the health of the animals but also for aesthetic reasons. We want … visitors [to] mentally transport themselves into the world beneath the water.”
Sally agrees that she spends much of her day cleaning tanks, noting that she’ll often get herself pretty filthy in the process. “There are so many ways to get covered in fish poop,” she jokes. On certain weekdays, she’ll clean the substrate and filters of her aquariums, and fecal matter collects in both locations. Then there’s the protein skimmer, a handy device used to filter out solid waste. “It’s basically just a cup of poop that you have to wash out in a sink,” she explains.

Nurse sharks, lemon sharks, and other good-sized species often share huge, expensive enclosures with various smaller fish. Ever wonder what stops the notorious predators from turning these tanks into sushi bars?
The answer is actually pretty straightforward. When a carnivore’s belly stays full, it loses the urge to hunt. So aquarists make sure that their sharks stay satisfied. At the Tennessee Aquarium, the biggest marine tank is a mixed-species setup that includes multiple sand tiger and sandbar sharks (both of which can grow to 8 feet or more). Three times every week, the predatory fish are given two to three percent of their body weight in food. A typical meal consists of some mackerel meat dipped into the tank at the end of a large pole.
The Toronto-based Ripley’s Aquarium of Canada maintains a similar tank, filled with its own sand tigers. Like their counterparts in Tennessee, the keepers here stand on platforms and hold out long sticks tipped with some tasty shark chow. Apparently, the system works very well. “People frequently lose their poles while feeding the sharks. I’ve even fallen in the tank,” aquarist Nicole Petrovskis told Toronto Life magazine. “When I fell, all the sharks heard the noise and flew to the other side … People ask if I was scared, but the first thing I thought of when I fell in was, ‘Damn, it’s weird to swim with shoes on.’”
All images via iStock unless otherwise noted.
* Some names have been changed.
September 29, 2016 – 12:00pm
The plight of the bumble bee is getting serious. The U.S. Fish and Wildlife Service just proposed that the rusty patched bumble bee (Bombus affinis) be officially classified as an endangered species.
According to the USFWS, the bee has seen a steep decline in population from the 1990s, when it could be found across 31 states, to a current low point of just 13. Possible factors include climate change, habitat loss, pesticides, and pathogens introduced by commercially produced bees that circulate in the wild.
The status of Bombus affinis is particularly concerning since the bees are usually found pollinating several key crops, including apples, cranberries, and plums.
The Service will be taking comments on the addition until November 21, with a final decision expected afterward. If the proposal is enacted, it would mark the first time any species of bee has been granted federal protection.
[h/t Seeker]
Know of something you think we should cover? Email us at tips@mentalfloss.com.
September 29, 2016 – 11:45am
As a recurring feature, our team combs the Web and shares some amazing Amazon deals we’ve turned up. Here’s what caught our eye today, September 29.
Mental Floss has affiliate relationships with certain retailers, including Amazon, and may receive a small percentage of any sale. But we only get commission on items you buy and don’t return, so we’re only happy if you’re happy. Good luck deal hunting!

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Philips Connected Health Watch, Black for $199.99 (list price $249.99)






iHome Bluetooth Rechargeable Mini Speaker Cube – Black for $19.99 (list price $24.99)





DeLonghi ESAM3300 Magnifica Super-Automatic Espresso/Coffee Machine for $499.95 (list price $699.95)

Estilo EST0286 Stainless steel Utensil Holder Jumbo – 7 X 7″ for $10.20 (list price $11.99)
KD Organizers 6-Slot Large Mesh Drawer Organizer for $24.97 (list price $37.45)

Hamilton Beach 25460A Panini Press Gourmet Sandwich Maker for $24.59 (list price $63.99)


Dash Go Rapid Egg Cooker, Black for $14.96 (list price $16.75)
Chef’s Choice 609 Premium Electric Food Slicer for $99.95 (list price $130.00)


Presto 06300 Dehydro Electric Food Dehydrator for $34.02 (list price $59.99)
RTIC 30 oz. Tumbler for $14.76 (list price $59.95)

Zyliss Chef’s Knife with Sheath Cover, 7.5-Inch Stainless Steel Blade for $12.89 (list price $13.99)


Pyrex Prepware 3-Piece Glass Mixing Bowl Set for $12.49 (list price $14.69)

Cuisinart AMB-14PP Chef’s Classic Nonstick Bakeware 14-Inch Pizza Pan for $10.80 (list price $45.30)


Lodge L9OG3 Pre-Seasoned Cast-Iron Round Griddle, 10.5-inch for $13.19 (list price $24.00)
Thirstystone Bamboo and Faux Leather Ambiance Coasters, Multicolor for $12.35 (list price $17.99)

Cuisinart CWO-25 Electric Wine Opener, Stainless Steel for $29.95 (list price $55.00)
IMUSA USA GAU-80305 1100W Electric Single Burner, Black for $11.99 (list price $15.99)

BLACK+DECKER TR1478BD 4-Slice Toaster, Black for $29.92 (list price $39.99)
Kitchen Supply 14-Inch x 16-Inch Aluminum Pizza Peel with Wood Handle for $22.95 (list price $28.50)


Rada Cutlery S01 Paring Knives Galore Gift Set for $14.29 (list price $20.60)

Simmons Curv 3-inch Flat Gel Memory Foam Mattress Topper – Queen for $139.99 (list price $139.99)

Hoover Sprint QuickVac Bagless Upright Vacuum, UH20040 – Corded for $47.29 (list price $79.99)

CapRack18 – Baseball Cap Holder (Black) for $10.00 (list price $15.00)
Remedy Complete Comfort Shredded Memory Foam Body Pillow for $19.00 (list price $29.81)


AmazonBasics Full/Queen Reversible Microfiber Comforter, Navy Blue for $19.03 (list price $24.99)
Saturday Knight Map of the World Peva Shower Curtain for $8.09 (list price $24.99)





Armor All 2.5 Gallon, 2 Peak HP, Utility Wet/Dry Vacuum, AA255 for $34.87 (list price $49.99)
Jokari 16930 Baggy Rack Storage Bag Opener and Holder for $3.99 (list price $9.99)


BioBag: Food Waste Certified Compostable, 3 Gallon, 25 ct (3 pack) for $14.59 (list price $23.94)
Whitmor 6044-14 White Crystal Collection Handbag File, Clear for $5.39 (list price $18.65)

Dreamfoam Bedding Arctic Dreams 10-Inch Cooling Gel Mattress, Queen for $279.99 (list price $799.99)
AmazonBasics 5-Shelf Shelving Unit – Black for $44.09 (list price $59.99)

Holmes 12 Inch Blizzard Remote Control Power Fan with Rotating Grill for $34.76 (list price $49.99)

DecoBros Two Tier Mesh Sliding Cabinet, Bronze (BK-017) for $23.87 (list price $29.99)

Ikea 172.283.40 Frakta Shopping Bag, Large, Blue, Set of 5 for $10.99 (list price $17.99)
InterDesign Axis Towel Holder for Bathroom Vanities – Bronze for $13.12 (list price $21.00)

Organize It All Metro 4-Tier Shelf (16984) for $27.41 (list price $60.84)
CreativeWare Complete Bath Towel Butler for $17.99 (list price $29.99)

Philips Connected Ear Thermometer, White, DL8740/37 for $47.99 (list price $59.99)

Philips Connected Body Analysis Weight Scale, White, DL8781/37 for $79.99 (list price $99.99)

Gillette Mach3 Turbo Cartridges 10 Count for $19.82 after on-screen coupon (list price $31.99)

Aquaphor Baby Wash & Shampoo 13.5 Fluid Ounce for $6.29 (list price $8.29)

Bed Head Deep Waver for $18.64 (list price $27.99)

Edge Shave Gel for Men Sensitive Skin – 7 Ounce (Pack of 6) for $16.02 (list price $26.99)

Burt’s Bees Renewal Night Cream, 1.8 Ounce for $14.19 (list price $19.99)
RUSK Engineering W8less Professional 2000 Watt Dryer for $79.95 (list price $135.95)

CHI Air Vibe Digital Touch Hair Dryer 1800W in Pink for $99.99 (list price $149.99)





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Smitco LLC Exfoliating Shower/bath Beauty Wash Gloves (4 Pairs) for $11.99 (list price $21.99)
Dove Beauty Bar, Sensitive Skin 4 oz, 16 Bar for $16.19 (list price $25.09)

Neutrogena T/Gel Therapeutic Shampoo Original Formula, 16 Fl. Oz for $13.99 (list price $16.39)

Origami RDE-01 Computer Desk for $109.99 (list price $149.99)

Oceanstar Portable 2-Tier Metal Rolling File Cart, Black for $31.87 (list price $39.99)

Casio Inc SL-100L Standard Function Calculator for $4.94 (list price $6.99)

E6000 230010 Craft Adhesive, 3.7 Fluid Ounces for $7.50 (list price $8.37)
Perler Beads 6,000 Count Bucket-Multi Mix for $6.84 (list price $10.69)

Paper Mate Flair Felt Tip Pens, Medium Point, Black, 12-Count for $8.70 (list price $20.99)


Scotch Precision Ultra Edge Scissors, 8 Inch, 3-Pack (1458-3AMZ) for $12.77 (list price $25.62)
Letraset Pro Marker Blending Set 1, Twin-Tip, 12-Pack for $24.57 (list price $29.95)

Pro Art 18-Piece Sketch/Draw Pencil Set for $6.26 (list price $9.95)

Enchanted Fairy Garden Kit for $19.90 (list price $25.99)

Scotch Cushion Wrap 7961, 12 Inches x 100 Feet for $15.44 (list price $18.99)


Fiskars 45mm Contour Rotary Cutter (195210-1001) for $11.18 (list price $17.00)
Tulip 29025 Dimensional Glow Fabric Paint, 6-Pack for $7.01 (list price $10.49)

Rubbermaid 5E28 Deluxe Tool Tower Rack with Casters, Holds 40 Tools for $37.99 (list price $99.99)
Deal of the Day: Save Up to 35% on ’47 NFL & NCAA Hats, Tees, and More

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Woodstock Famous Melodies, Pachelbel Canon Chime, Bronze for $38.00 (list price $48.35)

Fiskars Long Handle Digging Shovel (9668) for $21.99 (list price $34.99)

Heininger 5995 58,000 BTU Portable Propane Outdoor Fire Pit for $99.97 (list price $149.99)

Garrett Ace 400 Metal Detector for $339.95 (list price $399.95)

Igloo Products Corporation 00044669 Stainless Steel Cooler, 54 quart for $94.44 (list price $189.99)

Coleman 10 x 10 Instant Screened Canopy for $96.85 (list price $119.99)

Coleman 5693A703G Jug 1/2Gal Bail for $10.22 (list price $12.00)
Coleman Montana 8-Person Tent for $143.37 (list price $219.99)


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Adams Manufacturing 8500-48-3700 Plastic Quik-Fold Side Table, White for $13.81 (list price $24.99)

Great States 204-14 Hand Reel 14 Inch Push Lawn Mower for $44.49 (list price $87.90)
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Bosch GT3000 Glass and Tile Set, 8-Piece for $29.76 (list price $55.58)

Bosch HCBG800 Blue Granite 8-Piece Hammer Drill Bit Set for $30.74 (list price $38.18)

Hillman Fastener Corp 130251 Picture Hanger Assortment Kit for $8.84 (list price $9.99)

Bosch 24A ICON Wiper Blade – 24″ (Pack of 1) for $10.70 (list price $25.76)
Viking 862400 Blue Microfiber Wax Applicator, (Pack of 6) for $5.30 (list price $7.49)Sperry Instruments GFI6302 GFCI Outlet Tester for $7.99 (list price $13.99)
September 29, 2016 – 12:10pm
Japan Prepares for Halloween With Black Garlic Doritos

Let me put this right up front. The headline to this post is slightly misleading, but not dishonest. For the origin of the word slang indeed has been found, just not recently. It has been found for more than 100 years. The occasion for bringing this fact up now, and for the misleading headlinese, is that people still persist in presenting the origin as disputed. Anatoly Liberman, a highly respected etymologist who is in no way averse to hedging when warranted, tries to put the practice to rest in a recent Oxford University Press blog post titled “The Origin of the Word SLANG is Known!” That important exclamation point says not “exciting discovery!” but “Sheesh! Stop acting like it isn’t known already!”
As Liberman explains, it goes back to an old use of slang for “a narrow piece of land running up between other and larger divisions of ground.” It’s related to Scandinavian terms having to do with free movement and wandering, and the word’s “route was from ‘territory; turf’ to ‘those who advertise and sell their wares on such a territory,’ to ‘the patter used in advertising the wares,’ and to ‘vulgar language’ (later to ‘any colorful, informal way of expression’).”
Liberman has made his life’s work the thorough investigation of words marked “origin unknown” in etymological dictionaries. Though the explanation of slang laid out above appeared in his 2008 dictionary and is often credited to him, he gives credit to John Sampson, who set it forth in 1898. It was overlooked at the time, and Liberman laments,
“Few English words of disputable origin have been explained so convincingly, and it grieves me to see that some dictionaries still try to derive slang from Norwegian regional slengja ‘fling, cast’ or the phrase slengja kjeften ‘make insulting allusions’ (literarally ‘sling the jaw’), or from the old past tense of sling (that is, from the same grade of ablaut as the past tense of sling), or from language with s– appended to it (even if the amazing similarity between slang and language helped slang stay in Standard English, for many people must have thought of some hybrid like s-language). All those hypotheses lack foundation. The origin of slang is known, and the discovery made long ago should not be mentioned politely or condescendingly among a few others that stimulated the research but now belong to the museum of etymology.”
If you’re still not convinced, read the more involved explanation in his blog post or dictionary.
September 29, 2016 – 11:00am
Why Do Alligators Fall Asleep if You Rub Their Bellies?
Today’s Big Question: Why do alligators fall asleep when you rub their bellies?

Could a Fungus Cause Crohn’s Disease?
A recent study found that fungus may play a role in the development of Crohn’s disease.

Be grateful you weren’t living in Argentina 14,000 years ago: Archaeologists have discovered buried leftovers of ancient feasts in south Buenos Aires. Among them? Horses and sloths.
